FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23003038
Sequences flagged as poor quality0
Sequence length15-42
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTAC114700.062.50663830
CACCTTA115350.062.08228326
ACCTTAC116200.061.80286427
CTTACTA116100.061.6191729
TACTACC119800.059.8240531
CCTTACT120700.059.7520528
TACCAGA124050.057.89080434
ACTACCA124550.056.8364932
CCACCTT128750.055.4187225
CTACCAG128800.055.23897633
TCCACCT129950.054.9318524
CCAGACA139400.054.36898836
ACCAGAC145100.049.44619835
ACGAGTA21900.049.1423827
ACTCCAC136100.047.67331722
CTCCACC153600.046.3929223
TACGAGT24150.044.81941226
TTAGACG7450.044.53236434
AGTATAG20200.044.38300733
GTATAGG20800.043.36486434
Produced by FastQC (version 0.11.5)