FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16533499
Sequences flagged as poor quality0
Sequence length15-41
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCAG77600.074.6064533
CACCTTA70300.072.9571926
TACCAGA77900.072.92087634
ACCTTAC70600.072.9155827
TTACTAC69100.072.45357530
CTTACTA69500.071.6328629
TACTACC70900.071.1934931
CCTTACT73100.070.1187628
ACTACCA74250.068.63647532
CCACCTT80350.064.27260625
ACCAGAC93250.061.26116635
AGTATAG15150.056.23301333
GTATAGG15350.056.1070134
TATAGGC15050.055.7034735
ACTCCAC84650.055.54042422
ACGAGTA7850.054.85962727
CTCCACC100250.052.5202723
TCCACCT101500.050.6972624
CGTGCAA13150.048.0667133
AAGTATA16150.047.5115232
Produced by FastQC (version 0.11.5)