FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20200214
Sequences flagged as poor quality0
Sequence length15-42
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTTA274400.085.2269226
ACCTTAC275300.085.1711227
TTACTAC277500.084.4579530
CTTACTA278850.083.8997429
CCTTACT280550.083.7539128
CCAGACA286350.082.8719936
CTACCAG284850.082.559833
CCACCTT286350.081.5125925
ACTACCA288350.081.2505332
TACCAGA292550.081.0651634
TACTACC290300.080.7965731
TCCACCT292600.079.6356724
ACCAGAC303550.078.0451835
CTCCACC312150.074.549823
TTAGACG16300.073.4764334
ACTCCAC289900.069.7667622
TAGACGG18200.065.5487335
ATAGGCG42100.063.59434536
GTATAGG46450.057.76792534
AGTATAG46050.057.57942633
Produced by FastQC (version 0.11.5)