FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20712676
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT20250.017.5111751
CGTATCG16750.016.6002523
CCCGTAT22300.016.05731
TCGACGT17950.014.5224352
CCGTATC22500.013.9028122
CGCGTAT3556.5938366E-913.7100761
CCGATTG22400.013.344123
GCCGTTA13600.013.28903629
GTCCGTT22900.013.2076011
CCCGTCT64250.012.9319771
TACCGCG13200.012.63867722
CTCGACG23800.012.4160131
CCGTTAA14850.012.40444830
CGACGTC22100.011.9525583
CTATCGC9350.011.91504363
GTCGCAT12150.011.4452131
TCCGATT39500.011.2630372
ATCGCGC20800.010.94293867
CTCGTAT19800.010.5347991
CTCCGCT61150.010.0627571
Produced by FastQC (version 0.11.5)