FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18526114
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT19350.018.5077971
CGTATCG15600.018.049013
GTCCGAT17450.017.3349381
CCGTATC21150.016.4357992
TCGACGT17000.013.7002042
TATCGAG19550.013.1573527
TCCGATT35100.013.0727822
TACGACA19350.012.754619
TACCGCG11850.012.61368322
CCGATTG21250.012.1050263
CTACGAC20700.012.09070518
CCCGTCT55550.012.0175421
ATCGCGC21300.011.99170567
GCCGTTA12550.011.910130529
CTCCGAT30550.011.7226141
GTCACAT93850.011.5589611
GTCCGGT17800.011.5247221
TATCGCG4551.7003913E-811.45965722
CGACGTC21200.011.313743
CTCGACG23400.011.2926581
Produced by FastQC (version 0.11.5)