FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18060679
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG17250.018.94233
CCCGTAT22050.018.7650171
GTCCGAT16650.016.0800381
CCGTATC25250.015.2813432
TCGACGT18300.015.0063812
CTCGACG24100.014.2833061
CTCCGAT32600.013.7588671
TCCGATT33450.013.3019042
CCCGTCT58600.013.2911021
ATCGCGC19550.013.06595267
CCGATTG19950.012.8938553
CATCGCG22150.012.74494966
CGACGTC23400.012.3298043
CTCGTAT17800.012.1110641
TCGACCG7850.011.95615931
GTCCGTT17550.011.8873411
GTCGTTA16200.011.804791
GCCGTTA11850.011.73378129
TACATCG22700.011.49956964
CGCGCCA25750.011.35833169
Produced by FastQC (version 0.11.5)