FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25074596
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT23950.016.9852331
TATCGCG5400.016.7357522
CGTATCG20000.016.336963
CCCGTAT26650.015.3948691
TCGACGT21450.012.1538382
CCGTATC29200.012.1421832
CCGATTG29900.011.9740053
TATCGAG27650.011.8165687
GTCCGTT26300.011.7659081
GCCGTTA18200.010.69497229
TACGACA31000.010.65182519
TCCGATT47550.010.5997822
GATTGCG15850.010.55301870
TACCGCG18650.010.43697922
CTACGAC31000.010.42755618
GTCGAAT17250.010.2795061
AATATCG35600.010.2519335
GTCACAT122250.010.23871
GTCCAAT92400.010.1973781
GTCGGAT20500.010.0066651
Produced by FastQC (version 0.11.5)