FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20379848
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG15850.018.2041553
CCCGTAT22850.016.4348531
TACCGCG13800.014.61093722
CCGTATC24300.014.3060482
GTCCGAT20350.012.9860611
TCGACGT19850.012.7846352
GCCGTTA15850.012.50158729
CCGTTAA17250.012.09157230
GTCCGTT21700.011.8576951
CCGATTG23300.011.339123
ATCGCGC21500.011.06802367
CTCGACG27450.011.0205971
CCCGTCT60150.010.9836381
TCCGATT39800.010.9182462
CTATCGC8950.010.88682563
ATTATCG17650.010.83261410
CATCGCG23900.010.79081966
GCCGTAT13050.010.658051
CGACGTC23500.010.35504153
TACATCG25050.010.28139764
Produced by FastQC (version 0.11.5)