FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20116139
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT22750.020.480741
CGTATCG17050.019.3688963
CCGTATC23300.015.8148582
TCGACGT18800.014.6077932
GTCCGAT19550.014.0508491
ATCGCGC22200.013.87040767
TACCGCG15950.013.07651122
CATCGCG25700.012.61105666
CTCGTAT20500.012.551631
CTCGACG24700.012.5289681
CGCGTAA4205.187758E-912.41548935
GCCGTTA16100.012.30685529
TCCGATT40200.012.1929392
CCCGTCT62300.012.1672011
CCGTTAA16950.012.09989230
GTCGGAT14100.011.8370741
CTCCGAT36950.011.6688881
CCGATTG22950.011.6630923
CGCGCCA29900.011.6052169
TACATCG27650.011.45497564
Produced by FastQC (version 0.11.5)