FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20500534
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG15700.019.4803223
GTCCGAT18700.018.4037931
CCCGTAT22150.017.891421
CCGTATC23150.016.5143382
GTCCGTT22400.014.7431171
TACCGCG13350.014.31829622
TATCGAG22900.013.9622997
GCCGTTA13700.013.44513629
CTACGAC24650.013.2531718
TACGACA24250.013.18514519
CCGATTG22650.012.7357033
CCGTTAA14250.012.68234230
ATCGCGC22400.012.03861467
AATATCG29550.011.7611935
TCGCGTA7400.011.470448569
GTCGTTT24000.011.1530391
CTATCGC10450.010.99379363
TCGACGT17950.010.8428392
TCTACGA29550.010.82019217
CATCGCG24700.010.7301266
Produced by FastQC (version 0.11.5)