FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19144527
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT18800.017.9396131
CCCGTAT20650.017.0059361
CGTATCG16450.016.481873
TATCGAG20550.013.70060357
CCGATTG21300.013.2185333
CCGTATC23650.012.7871532
TCGACGT16850.012.5839132
TACGACA23550.012.39835119
CTACGAC24200.012.06533718
GTCACAT95050.011.77885151
GTCGCAT12400.011.7767871
AATATCG26600.011.6299925
TCGCGTA8650.011.591125569
TCTACGA25950.011.25150617
GTCCGGA26750.010.9182921
GCCGTTA13400.010.89467729
GTCCGTT22200.010.8067551
CCCGTCT57700.010.7863971
CTCGACG23100.010.68674851
GTCGCTT20250.010.6454951
Produced by FastQC (version 0.11.5)