FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20736766
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG17350.020.6375063
CCCGTAT23700.019.6598241
GTCCGAT18800.019.4202421
CCGTATC27000.016.737992
GCCGTTA14100.013.8066229
TCCGATT41200.013.6691492
TACCGCG14000.013.65706322
CCGTTAA14600.013.5718630
GTCCGTT21800.013.5576881
ATCGCGC24750.013.00510867
CCGATTG22850.012.93163
TCGACGT19750.012.8492992
CCCGTCT63350.012.241
CATCGCG28350.011.9235566
CGACGTC22950.011.5119913
ATCGAAG39800.011.179936
CGCGCCA31100.011.14332469
GCGCCAT45350.011.11805470
GTATCGA32450.011.0340434
TCGCGCC29600.010.96296268
Produced by FastQC (version 0.11.5)