FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23043991
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT25350.022.7699011
CGTATCG18200.022.5376573
CCGTATC26850.017.8666572
TCGACGT22150.016.9495642
GTCCGAT21550.015.9741941
GTCCGTT22850.015.2175521
ATCGCGC25200.014.99315867
CTCGACG28850.014.3427411
CGACGTC25850.013.7163243
TACCGCG14350.013.323153522
CGCGCCA31850.013.18133969
TACATCG29600.013.16844764
CATCGCG29400.013.03906666
TCCGATT41650.012.7698232
TCGCGCC30450.012.65153168
CTCGTAT19250.012.4637681
ATCGAAG43900.012.3523816
CCCGTCT72300.012.3121151
GCCGTTA14900.012.13146729
CGTGTTA31250.011.798923559
Produced by FastQC (version 0.11.5)