FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22148934
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG19000.022.5016353
CCCGTAT36000.017.9634081
CCGTATC27450.017.7277722
TCGACGT20450.016.48722
CGCGAGA22050.015.92632552
CGTCCGT24000.015.41946569
ATCGCGT17350.015.25036564
TATCGCG17850.014.82053763
CTCGACG25400.014.78319551
ATCGCGC26300.014.6464867
CTCCGAT39450.014.2773071
GTCCGAT21000.014.0727161
CCTATCG19350.013.6676461
CGCGTAT12550.013.5747131
TCGCACG19500.013.54658410
TCCGATT42600.013.5446232
CCCGCAT42250.013.49571
TACGTCC27100.013.43879667
CCCGTCT74850.013.3776231
CGCGTTA19600.013.34148566
Produced by FastQC (version 0.11.5)