FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20771012
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT23250.021.2347581
CGTATCG17200.020.6135753
CCGTATC25050.017.7623122
TCGACGT20900.016.4659272
GTCCGAT18500.015.0348891
CGACGTC23750.014.9285683
CTCGACG26150.014.4922831
ATCGCGC24550.012.97733667
GCCGTTA12200.012.8211529
TACCGCG12550.012.74067922
CCCGTCT66100.012.7290461
GTCCGTT22500.012.362021
GTCCGGT20650.012.2909311
CCGTTAA14450.011.78700730
CATCGCG28400.011.78545866
CCGATTG22750.011.6121813
GTTCGAT21750.011.5094661
TACATCG28500.011.48376964
TCCGATT38750.011.3030622
CGTGTTA29050.010.898952559
Produced by FastQC (version 0.11.5)