FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16831045
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACC908350.5396872267883545No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC885480.5260992410156351No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG673030.39987416111120844No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA648940.3855613243265644No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA544270.32337267234446826No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG494610.29386767131809105No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCCTTCTATAAA485010.28816392565048693No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC460160.2733995423338242No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT289670.17210458411821725No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAGGCGTGCAAGTC287220.17064894069263079No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACCAAACAAAAAGAGC220530.1310257325079934No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG196200.11657030208165924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCC402550.055.234063
AGTATGG398700.054.962841
GTATGGC410600.054.2120442
TGGCCCG421200.052.8214045
CCGGGGG424650.052.3840759
CGGGGGA429600.051.8209510
ACTTACC145750.050.65126869
CCCGGGG441150.050.4957168
ATGGCCC448000.049.6693084
GGGGATC448550.049.6239112
GGGATCC456450.048.7269613
GCCCGGG460950.048.304067
GGCCCGG461000.048.298826
TAATATG116300.047.91021770
TCCTACG130950.047.3595617
GATCCTA219100.046.98014515
GGATCCG153400.046.32056414
ATCCTAC136350.045.61141216
TACTGGA486800.044.3763852
GGATCCT343750.044.18325414
Produced by FastQC (version 0.11.5)