FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004955982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15594352
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACC456290.2925995257770249No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC438290.28105688521074806No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG342240.21946407263347653No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA342100.21937429654018326No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA287680.18447704656147304No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG263690.16909327171786298No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCCTTCTATAAA256660.16458522931892267No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC241710.1549984250708205No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACCAAACAAAAAGAGC165490.10612175485073057No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT158780.10181891495074627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)