FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14395552
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACC854450.5935513969870693No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC838310.5823396004543626No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA630250.4378088453989121No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG625800.43471761277372345No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA508060.35292846012434953No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG451960.3139580892764654No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCCTTCTATAAA449650.3123534269474349No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC415350.28852662266789075No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT239300.1662319027432918No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAGGCGTGCAAGTC236350.16418265864344764No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACCAAACAAAAAGAGC216890.15066459417464506No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG200710.13942501128126245No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG173580.12057891215286498No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT170970.11876585211876559No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG169060.11743905339649359No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC154200.10711642040541411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATGG373100.054.9054151
TATGGCC380150.054.6313553
GTATGGC384400.054.082742
CGGGGGA397800.052.25075510
TGGCCCG403700.051.4786875
CCGGGGG406700.051.073139
ACTTACC137550.050.43222869
GGGGATC415100.050.08986712
TAATATG102200.049.69996670
CCCGGGG420600.049.4596678
GGGATCC421800.049.26125313
ATGGCCC423750.049.059354
GCCCGGG437850.047.4555137
GGCCCGG444500.046.784826
TCCTACG128050.046.2691417
GATCCTA213550.046.04487215
GGATCCT320450.045.9401114
GGATCCG136700.044.61311314
AACTTAC144650.044.50531468
CATACTA125850.043.6798975
Produced by FastQC (version 0.11.5)