FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14858128
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACC711910.47913842174465043No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC687390.46263566985019916No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA514830.34649721687685014No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG514010.3459453303942462No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA434010.29210274672556324No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG392200.2639632664357179No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCCTTCTATAAA382010.25710506734091937No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC355800.2394648908664672No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAGGCGTGCAAGTC217620.14646528822473465No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT216320.14559034624011855No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG191570.12893279691761977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCC325550.053.730443
AGTATGG324600.053.1666641
GTATGGC330200.052.9855652
TGGCCCG342000.051.0947045
CGGGGGA344250.050.7504810
ACTTACC108500.050.6267769
CCGGGGG348350.050.1032649
CCCGGGG356850.048.9682778
GGGGATC357600.048.87529412
TCCTACG106650.048.27094717
GGGATCC363400.048.00913613
ATGGCCC364350.047.9604454
GCCCGGG373100.046.9659427
GGCCCGG376000.046.5667276
GATCCTA182200.046.5132815
TACGTTC115000.044.97764220
CGTATAA114750.044.96051842
TAATATG91200.044.67211570
GGATCCT276700.044.6477814
TCGTATA116050.044.60605641
Produced by FastQC (version 0.11.5)