FastQCFastQC Report
Wed 8 May 2024
EGAF00004955989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17094748
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT717460.41969615463182025No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC711470.4161921544558598No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA521470.30504690680435886No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG519830.3040875478246301No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA412580.24134898040029606No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA385350.22542011148687305No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG373210.21831851513692976No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC342570.20039488151565613No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCGCTATCTCGTAT265140.15510026822273135TruSeq Adapter, Index 3 (97% over 37bp)
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG231320.13531641414076417No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT217320.12712676431381148No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA216080.12640139532913852No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT209220.12238846691393171No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC183090.10710307048691212No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG180510.10559383501880226No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT172390.10084383811916971No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG172040.10063909687349588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)