FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004955992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004955992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13224319
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC972710.7355463823883861No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACC972290.7352287856939931No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA726670.5494952140824794No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG722520.5463570562688331No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA537620.4065388924752949No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG491130.37138396313640043No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCCTTCTATAAA485670.36725520610929No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC444420.3360626736242524No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT266680.201658777287511No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAGGCGTGCAAGTC260940.19731828913080515No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG241220.18240636814644293No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG173780.1314094132181778No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACCAAACAAAAAGAGC149160.11279219746589597No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT146440.11073538077839774No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG133630.10104868159940789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCC399350.057.397153
GTATGGC403450.056.8142852
AGTATGG399100.056.5487481
ACTTACC143500.056.35359269
TGGCCCG417650.054.857015
CGGGGGA419250.054.53963510
CCGGGGG420550.054.379319
TAATATG96900.053.5209670
GGGGATC435100.052.60878412
CCCGGGG436500.052.495818
ATGGCCC438950.052.2584534
GGGATCC441800.051.7952213
GCCCGGG451050.050.756147
TCCTACG139200.050.4586517
GGCCCGG455950.050.2566266
GATCCTA223400.049.68247215
ATCCTAC142050.049.47075716
AACTTAC152650.049.09339568
GGATCCT334900.049.00612314
GTATTAC122950.047.8260769
Produced by FastQC (version 0.11.5)