FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004956033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004956033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6365016
Sequences flagged as poor quality0
Sequence length18-20
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTTATCTAGCTGTAT67859310.66129291740979No Hit
GAGATGAAGCACTGTAGCTC4610907.244129472730313No Hit
TCAAGTAATCCAGGATAGGC3526235.540017495635518No Hit
GTAAACATCCTCGACTGGAA2067903.24885279157193No Hit
CCCTGAGACCCTAACTTGTG1825882.8686180835994755No Hit
AGCACCATCTGAAATCGGTT1512132.3756892362878586No Hit
GAGGTAGTAGATTGTATAGT1392392.187567164010271No Hit
GAGGTAGTAGGTTGTATAGT1330302.0900183126012566No Hit
CACAGTGAACCGGTCTCTTT1318242.0710709918089756No Hit
ACCCGTAGATCCGATCTTGT1022051.6057304490672135No Hit
TAAAGCTAGATAACCGAAAG1006821.581802779443131No Hit
ACATTCAACGCTGTCGGTGA1002711.575345607929344No Hit
GAGGTAGTAGTTTGTACAGT1000871.572454806083755No Hit
GCAGCATTGTACAGGGCTAT955721.5015201847096693No Hit
AAGGCACGCGGTGAATGCCA887071.393664996285948No Hit
GTAAACATCCCCGACTGGAA865361.3595566766839235No Hit
GTAAACATCCTTGACTGGAA833241.3090933314228903No Hit
AGCTTATCAGACTGATGTTG775081.2177188556949425No Hit
AACCGTTACCATTACTGAGT731651.1494865056112977No Hit
TACAGTACTGTGATAACTGA678441.0658889152831665No Hit
AAAGCTAGATAACCGAAAGT658881.0351584347941938No Hit
TCACAGTGGCTAAGTTCTGC648021.0180964195533837No Hit
GTAAACATCCTACACTCTCA618630.9719221444219465No Hit
ACCCGTAGATCCGAACTTGT604700.9500368891452905No Hit
GGAAGACTAGTGATTTTGTT541210.8502885145928933No Hit
CCCTGAGACCCTTTAACCTG517030.8122996077307583No Hit
CGTACCGTGAGTAATAATGC484720.761537755757409No Hit
TAAGGCACGCGGTGAATGCC425570.6686079029494977No Hit
TCAAGTAATTCAGGATAGGT400400.6290636190073992No Hit
ACAGTACTGTGATAACTGAA368160.578411743191219No Hit
CGAGGAGCTCACAGTCTAGT351620.5524259483401142No Hit
GGCTCAGTTCAGCAGGAACA342440.5380033608713631No Hit
AGCAGCACGTAAATATTGGC326910.5136043648594127No Hit
TTTGGTTATCTAGCTGTATG323600.5084040637132726No Hit
GAGATGAAGCACTGTAGCTT313220.4920961706930509No Hit
GAGGTAGTAGTTTGTGCTGT280100.4400617374724588No Hit
TCACATTGCCAGGGATTACC278670.4378150816902895No Hit
ACCCGTAGAACCGACCTTGC277830.43649536780426007No Hit
GAGGTAGTAGGTTGTGTGGT253750.3986635697380808No Hit
AACGGAATCCCAAAAGCAGC244050.38342401653035907No Hit
AGCTGCCAGTTGAAGAACTG241250.37902497024359405No Hit
AAGGCACGCGGTGAATGCCC229030.3598262753777838No Hit
TGTGCTTGATCTAACCATGT222680.3498498668345845No Hit
CCACAGGGTAGAACCACGGA213370.3352230379310908No Hit
GCTACATTGTCTGCTGGGTT211330.3320180184935906No Hit
TATAAAGCAATGAGACTGAT206110.32381693934469297No Hit
GAATTGTGGCTGGACATCTG198650.31209662316638326No Hit
GAGATGAAGCACTGTAGCTA193300.3036913025827429No Hit
TCACATTGCCAGGGATTTCC189580.29784685537318367No Hit
ACATTCATTGCTGTCGGTGG189230.297296974587338No Hit
GTAAACATCCTACACTCAGC188850.29669996116270564No Hit
ATTATTACTTTTGGTACGCG186120.29241089103310974No Hit
CGGATCCGTCTGAGCTTGGC180530.28362850933917527No Hit
ATTGCACTTGTCCCGGCCTG172020.27025855080332867No Hit
GAGGTAGTAGGTTGTATGGT170970.2686089084457918No Hit
CAAGAGCAATAACGAAAAAT166000.2608006012867839No Hit
AGCACCATTTGAAATCGGTT163730.2572342316185851No Hit
ACCACAGGGTAGAACCACGG162750.25569456541821733No Hit
CTACAGTGCACGTGTCTCCA161230.2533065117196877No Hit
TCCAGTTTTCCCAGGAATCC152660.23984228790626763No Hit
TAGCACCATCTGAAATCGGT148490.23329085111490686No Hit
GAGACCTCTGGGTTCTGAGC147190.23124843676748025No Hit
CCAGCATCAGTGATTTTGTT145980.22934742033641392No Hit
ATCGTACAGGGTCATCCACT135720.21322805787133922No Hit
AAGGCACGCGGTGAATGCCT134730.21167268079137586No Hit
CTTTGGTTATCTAGCTGTAA133150.20919036181527273No Hit
ATTGCACTCGTCCCGGCCTC130920.20568683566545629No Hit
GTACCGTGAGTAATAATGCG125320.19688874309192625No Hit
GAGGTAGTAAGTTGTATTGT122570.19256825120313917No Hit
TTTTTGCGGTCTGGGCTTGC117600.18475994404413124No Hit
GAGAACTGAATTCCATAGGC112050.1760404058685791No Hit
ATGGCTTTTTATTCCTATGT107610.16906477532813743No Hit
TCAACGGGTATTTATTGAGC106600.16747797648898288No Hit
CAGTGCATCACAGAACTTTG103680.1628903996470708No Hit
GGTAGACTATGGAACGTAGG100860.1584599316011146No Hit
GCTCGGTCTGAGGCCCCTCA99480.15629183021692325No Hit
AAGGCACGCGGTGAATGCCG98450.15467360961857754No Hit
CCCTGAGACCCTAACTTGTC96030.15087157675644491No Hit
TCACAGTGGCTAAGTTCTGA95500.15003890013787868No Hit
GAGATGAAGCACTGTAGCTG90180.1416807121930251No Hit
GGAATGTAAAGAAGTATGTA89900.14124080756434862No Hit
TATAATACAACCTGATAAGT85230.13390382679320836No Hit
CGTACCGTGAGTAATAATGA82840.13014892656986252No Hit
AGCACCATTTGAAATCAGTG82080.12895489972059773No Hit
GAGGTAGTAGGTTGCATAGT82080.12895489972059773No Hit
TAGACTGAAGCTCCTTGAGG81660.12829504277758297No Hit
AGCAGCACAGAAATATTGGC79760.12530997565442098No Hit
GCTACATCTGGCTACTGGGT77700.12207353445772956No Hit
GAGGTAGGAGGTTGTATAGT75420.11849145390993518No Hit
AAAGAATTCTCCTTTTGGGC74810.11753309025460423No Hit
ATGACACGATCACTCCCGTT74620.11723458354228804No Hit
TCACAGTGGCTAAGTTCTGT72970.11464228840901579No Hit
CCCTGAGACCCTAACTTGTT72630.11410811850276574No Hit
CTCACACAGAAATCGCACCC70550.11084025554688316No Hit
AGAAAGCTCACAAGAACTGC69840.10972478309559631No Hit
ACAATATCCTGGTGCTGAGT69540.10925345670772862No Hit
ATTGCACTTGTCTCGGTCTG69430.10908063703217714No Hit
AAAGTGCTGTTCGTGCAGGT69300.10887639559743448No Hit
AAAGCTGGGTTGAGAGGGCG65780.103346165979787No Hit
CTGGCTCCGTGTCTTCACTC65080.10224640440809575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)