FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004956034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004956034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401910
Sequences flagged as poor quality0
Sequence length18-20
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTTATCTAGCTGTAT67267112.452465887065872No Hit
TCAAGTAATCCAGGATAGGC2929055.422248797184699No Hit
CCCTGAGACCCTAACTTGTG2244144.154345407457733No Hit
AGCACCATCTGAAATCGGTT1372252.5403051883500467No Hit
GAGGTAGTAGGTTGTATAGT1282442.3740491789015365No Hit
GAGGTAGTAGATTGTATAGT1256542.3261031746178666No Hit
GTAAACATCCTCGACTGGAA1198052.2178266576081422No Hit
GAGATGAAGCACTGTAGCTC1186992.1973524179410617No Hit
TAAAGCTAGATAACCGAAAG1134182.0995907003263663No Hit
ACCCGTAGATCCGATCTTGT1007981.8659696292607615No Hit
GAGGTAGTAGTTTGTACAGT951131.7607290754566443No Hit
AAGGCACGCGGTGAATGCCA935861.7324612960971213No Hit
GCAGCATTGTACAGGGCTAT916801.6971774798173238No Hit
ACATTCAACGCTGTCGGTGA914011.692012639973639No Hit
GTAAACATCCCCGACTGGAA776721.437861793328656No Hit
ACCCGTAGATCCGAACTTGT698311.2927094305532671No Hit
CCCTGAGACCCTTTAACCTG683211.2647563546967646No Hit
AAAGCTAGATAACCGAAAGT663091.2275102695157822No Hit
GGAAGACTAGTGATTTTGTT633931.1735293627624304No Hit
GTAAACATCCTACACTCTCA614741.1380048908626765No Hit
GTAAACATCCTTGACTGGAA598901.1086819291694976No Hit
TACAGTACTGTGATAACTGA513890.9513116656886176No Hit
AGCTTATCAGACTGATGTTG503590.9322443357997449No Hit
TAAGGCACGCGGTGAATGCC475420.8800961141522166No Hit
CGTACCGTGAGTAATAATGC474510.8784115248125199No Hit
AACCGTTACCATTACTGAGT443710.8213946548535611No Hit
CGAGGAGCTCACAGTCTAGT381540.7063057326019871No Hit
TCAAGTAATTCAGGATAGGT335340.6207804276635486No Hit
TTTGGTTATCTAGCTGTATG322070.5962150424572049No Hit
GAGGTAGTAGTTTGTGCTGT306520.567428927916237No Hit
GGCTCAGTTCAGCAGGAACA306470.5673363680624075No Hit
AGCAGCACGTAAATATTGGC304040.5628379591662949No Hit
ACCCGTAGAACCGACCTTGC302720.5603943790251966No Hit
TCACATTGCCAGGGATTACC288650.5343480361575813No Hit
TGTGCTTGATCTAACCATGT272230.5039513801599804No Hit
AACGGAATCCCAAAAGCAGC266520.493381044852654No Hit
GAGGTAGTAGGTTGTGTGGT266160.4927146139050817No Hit
ACAGTACTGTGATAACTGAA260840.4828662454576252No Hit
GAATTGTGGCTGGACATCTG251940.46639059147597794No Hit
AAGGCACGCGGTGAATGCCC243210.45022964099735097No Hit
GCTACATTGTCTGCTGGGTT234030.43323565183425866No Hit
ACATTCATTGCTGTCGGTGG218430.4043569774394612No Hit
AGCTGCCAGTTGAAGAACTG199090.3685548259782188No Hit
TATAAAGCAATGAGACTGAT197610.3658150543048662No Hit
CGGATCCGTCTGAGCTTGGC193970.3590766969460802No Hit
CAAGAGCAATAACGAAAAAT181510.33601078137177404No Hit
AAGGCACGCGGTGAATGCCT176560.3268473558426556No Hit
CCAGCATCAGTGATTTTGTT174960.3238854405201123No Hit
GAGGTAGTAGGTTGTATGGT170930.31642511630145637No Hit
ATTGCACTTGTCCCGGCCTG166470.3081687773398668No Hit
GAGACCTCTGGGTTCTGAGC164690.3048736465435374No Hit
GTAAACATCCTACACTCAGC164190.3039480480052426No Hit
ATCGTACAGGGTCATCCACT155390.2876575137312543No Hit
TAGCACCATCTGAAATCGGT140450.2600006294070061No Hit
AGCACCATTTGAAATCGGTT138380.2561686514584656No Hit
CCACAGGGTAGAACCACGGA137510.2545581100018327No Hit
GAGAACTGAATTCCATAGGC135960.25168875453311884No Hit
CCCTGAGACCCTAACTTGTC135520.2508742278194194No Hit
GAGGTAGTAAGTTGTATTGT133480.2470977857831767No Hit
CTTTGGTTATCTAGCTGTAA123670.22893754246183293No Hit
GTACCGTGAGTAATAATGCG120550.22316180758287346No Hit
TTTTTGCGGTCTGGGCTTGC120150.2224213287522376No Hit
ACCACAGGGTAGAACCACGG119760.22169936189236772No Hit
ATTATTACTTTTGGTACGCG115290.21342451096001228No Hit
AAGGCACGCGGTGAATGCCG107490.1989851737626136No Hit
TCAACGGGTATTTATTGAGC107340.19870749420112516No Hit
CCCTGAGACCCTAACTTGTT103520.19163592136855298No Hit
TCACATTGCCAGGGATTTCC103210.1910620502748102No Hit
GAGATGAAGCACTGTAGCTT102840.19037710735647206No Hit
CTCACACAGAAATCGCACCC101810.1884703743675848No Hit
ATTGCACTCGTCCCGGCCTC96650.1789181974523826No Hit
GCTACATCTGGCTACTGGGT96090.1778815270894924No Hit
CAGTGCATCACAGAACTTTG95330.17647461731128436No Hit
ACAATATCCTGGTGCTGAGT94850.17558604271452136No Hit
GGTAGACTATGGAACGTAGG94830.17554901877298956No Hit
GGAATGTAAAGAAGTATGTA91570.16951411630330754No Hit
ATGGCTTTTTATTCCTATGT88960.16468249193340875No Hit
GAGGTAGGAGGTTGTATAGT87860.16264617514916022No Hit
GCTCGGTCTGAGGCCCCTCA87080.16120224142942033No Hit
CTGGCTCCGTGTCTTCACTC86560.16023961894959374No Hit
GAGGTAGTAGGTTGCATAGT85860.15894378099598105No Hit
AGCACCATTTGAAATCAGTG81760.1513538729819638No Hit
TAGACTGAAGCTCCTTGAGG79850.1478180865656777No Hit
TATAATACAACCTGATAAGT79760.14765147882878465No Hit
AACAGTCTACAGCCATGGTC78650.1455966500737702No Hit
ATGACACGATCACTCCCGTT75360.1395062116917905No Hit
GCTGGTGTTGTGAATCAGGC73410.13589637739244081No Hit
AAAGAATTCTCCTTTTGGGC72710.1346005394388281No Hit
AACAAACATGGTGCACTTCT71950.13319362966062004No Hit
CCCTGAGACCCTAACTTGTA66160.12247519858716639No Hit
AGAAAGCTCACAAGAACTGC65200.1206980493936404No Hit
CGTACCGTGAGTAATAATGA63550.1176435742172676No Hit
AGCAGCACAGAAATATTGGC62520.11573684122838032No Hit
TTGGTTATCTAGCTGTATGA62310.11534808984229653No Hit
ATAATACATGGTTGATCTTT59490.11012771408631392No Hit
GAGATGAAGCACTGTAGCTA59460.11007217817401622No Hit
TCCCTTTGTCATCCTATGCC58980.10918360357725324No Hit
ATTGCACTTGTCTCGGTCTG58460.10822098109742664No Hit
GGCAGTGTCTTAGCTGGTTG58380.1080728853312995No Hit
TATCAGAATCTCCAGGGGTA56990.10549972139483998No Hit
ACACACCTGGTTAACCTCTT56050.10375959614284577No Hit
AAAGTGCTGTTCGTGCAGGT55280.1023341743938718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)