FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004956036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004956036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5536180
Sequences flagged as poor quality0
Sequence length18-20
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTTATCTAGCTGTAT60037610.844589590656373No Hit
TCAAGTAATCCAGGATAGGC3252765.8754592516861806No Hit
CCCTGAGACCCTAACTTGTG2191353.9582347394774016No Hit
CACAGTGAACCGGTCTCTTT2013023.63611732277491No Hit
AAGGCACGCGGTGAATGCCA1542462.786144959159565No Hit
AGCACCATCTGAAATCGGTT1541592.7845734784634892No Hit
GAGGTAGTAGGTTGTATAGT1528032.7600800552005174No Hit
GAGGTAGTAGATTGTATAGT1516982.740120444060706No Hit
GAGGTAGTAGTTTGTACAGT1090661.9700587769906326No Hit
GCAGCATTGTACAGGGCTAT1057081.9094032347214143No Hit
ACCCGTAGATCCGATCTTGT908991.641908319454931No Hit
TAAAGCTAGATAACCGAAAG906891.6381150901885415No Hit
GTAAACATCCTCGACTGGAA841561.5201095340108162No Hit
TAAGGCACGCGGTGAATGCC778381.4059875220820133No Hit
CCCTGAGACCCTTTAACCTG753411.360884219804992No Hit
GTAAACATCCCCGACTGGAA724561.3087724748834033No Hit
GGAAGACTAGTGATTTTGTT684661.2367011188220036No Hit
ACATTCAACGCTGTCGGTGA669991.2102027029467972No Hit
AAAGCTAGATAACCGAAAGT588951.0638201792571773No Hit
GAGATGAAGCACTGTAGCTC541880.9787976547005336No Hit
GTAAACATCCTACACTCTCA521080.9412266219667713No Hit
TACAGTACTGTGATAACTGA450120.8130515987558209No Hit
GTAAACATCCTTGACTGGAA400020.7225559862576723No Hit
AAGGCACGCGGTGAATGCCC367350.6635441766705562No Hit
TCACAGTGGCTAAGTTCTGC344960.623101127492242No Hit
GAGGTAGTAGTTTGTGCTGT341490.616833267704446No Hit
AACCGTTACCATTACTGAGT329430.5950492939174666No Hit
AAGGCACGCGGTGAATGCCT318740.5757399506518935No Hit
CGTACCGTGAGTAATAATGC309500.55904974187978No Hit
GCTACATTGTCTGCTGGGTT289950.5237365837093447No Hit
AGCTTATCAGACTGATGTTG284550.5139825655957718No Hit
ACCCGTAGATCCGAACTTGT283740.5125194628787358No Hit
TCAAGTAATTCAGGATAGGT281920.5092319975145316No Hit
CGAGGAGCTCACAGTCTAGT270200.4880621656087772No Hit
GAGGTAGTAGGTTGTATGGT267390.48298646359041797No Hit
AACGGAATCCCAAAAGCAGC264620.4779830135580852No Hit
TTTGGTTATCTAGCTGTATG261720.4727447445711664No Hit
AGCAGCACGTAAATATTGGC249530.4507259518296009No Hit
CTACAGTGCACGTGTCTCCA249030.4498228020042701No Hit
TGTGCTTGATCTAACCATGT243790.4403577918348031No Hit
ACAGTACTGTGATAACTGAA241820.43679938152299963No Hit
GAGGTAGTAGGTTGTGTGGT224480.40547814558052664No Hit
GGCTCAGTTCAGCAGGAACA214460.38737902308089694No Hit
TCACATTGCCAGGGATTACC204040.3685573807210025No Hit
GAGGTAGTAAGTTGTATTGT202040.36494478141967923No Hit
AGCACCATTTGAAATCGGTT188280.34009009822657504No Hit
ATCGTACAGGGTCATCCACT187560.3387895624780986No Hit
CAAGAGCAATAACGAAAAAT186040.33604398700909294No Hit
ACCCGTAGAACCGACCTTGC185330.33476151425712314No Hit
GCTACATCTGGCTACTGGGT171370.30954557113388653No Hit
AAGGCACGCGGTGAATGCCG162590.2936862602010773No Hit
AGCTGCCAGTTGAAGAACTG154910.27981387888399584No Hit
GTAAACATCCTACACTCAGC152140.27481042885166307No Hit
CGGATCCGTCTGAGCTTGGC148170.2676394192385363No Hit
TATAAAGCAATGAGACTGAT142620.25761445617736417No Hit
GAGACCTCTGGGTTCTGAGC141020.25472437673630555No Hit
CCCTGAGACCCTAACTTGTC140270.2533696519983093No Hit
ACATTCATTGCTGTCGGTGG134780.24345306691617685No Hit
TAGCACCATCTGAAATCGGT130670.2360291753519575No Hit
ATGGCTTTTTATTCCTATGT120660.2179481158488344No Hit
CTTTGGTTATCTAGCTGTAA117040.21140931111343922No Hit
GAGGTAGTAGGTTGCATAGT114050.20600847515796092No Hit
TCAACGGGTATTTATTGAGC112700.20356997062956766No Hit
GAGGTAGGAGGTTGTATAGT109890.19849426861120847No Hit
AGCACCATTTGAAATCAGTG109130.1971214808767056No Hit
GAGAACTGAATTCCATAGGC104710.18913763642078113No Hit
ATTGCACTTGTCCCGGCCTG101540.1834116665281837No Hit
CTGGCTCCGTGTCTTCACTC101070.18256270569237273No Hit
AACAAACATGGTGCACTTCT96090.1735673334320777No Hit
CCCTGAGACCCTAACTTGTT94850.17132752186525727No Hit
GCTGGTGTTGTGAATCAGGC94380.17047856102944628No Hit
GGTAGACTATGGAACGTAGG90190.162910165493174No Hit
TTTTTGCGGTCTGGGCTTGC88020.1589904952512382No Hit
CCAGCATCAGTGATTTTGTT83120.15013962696299615No Hit
CTCACACAGAAATCGCACCC82640.14927260313067856No Hit
GTACCGTGAGTAATAATGCG81200.1466715316337258No Hit
TATTGCTTAAGAATACGCGT78220.14128875867475407No Hit
AAGGCACGCGGTGAATGCTT77240.13951858501710565No Hit
GCTCGGTCTGAGGCCCCTCA76020.13731489944329844No Hit
ATAATACATGGTTGATCTTT75280.13597823770180884No Hit
ATTATTACTTTTGGTACGCG72920.13171537052624735No Hit
CCACAGGGTAGAACCACGGA72420.13081222070091653No Hit
TCCCTTTGTCATCCTATGCC71420.12900592105025485No Hit
CAAGTAATCCAGGATAGGCT69400.12535719575591833No Hit
ATTGCACTCGTCCCGGCCTC68350.12346058112272362No Hit
CAGTGCATCACAGAACTTTG67500.12192522641966122No Hit
TCTCACTACTGCACTTGACT67150.12129302154192964No Hit
GGAATGTAAAGAAGTATGTA67000.1210220765943304No Hit
AGCAGCACAGAAATATTGGC65570.11843906809388421No Hit
TCACATTGCCAGGGATTTCC65260.11787911520217911No Hit
ACCACAGGGTAGAACCACGG65170.11771654823361957No Hit
ATGACACGATCACTCCCGTT63080.11394138196373672No Hit
GAATTGTGGCTGGACATCTG62060.11209895632006185No Hit
ACACACCTGGTTAACCTCTT60700.109642388795162No Hit
TAAGGCACGCGGTGAATGCT59590.10763739618292757No Hit
AGAAAGCTCACAAGAACTGC59560.10758320719340772No Hit
ACATTCAACCTGTCGGTGAG59200.10693293931916953No Hit
TATCAGAATCTCCAGGGGTA58270.1052530806440542No Hit
AAGGCACGCGGTGAATGCTC58210.10514470266501451No Hit
CCCTGAGACCCTAACTTGTA58040.10483763172440203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)