FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004956037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004956037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6122342
Sequences flagged as poor quality0
Sequence length18-20
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTTATCTAGCTGTAT63112010.3084734567262No Hit
TCAAGTAATCCAGGATAGGC4061656.634144253947263No Hit
CCCTGAGACCCTAACTTGTG2496654.077932921747919No Hit
GAGGTAGTAGGTTGTATAGT1844843.013291318910312No Hit
ACATTCAACGCTGTCGGTGA1664032.7179631585429234No Hit
GAGGTAGTAGATTGTATAGT1630672.6634742064393No Hit
GAGATGAAGCACTGTAGCTC1592542.6011941181985585No Hit
CACAGTGAACCGGTCTCTTT1425622.32855335425561No Hit
AGCACCATCTGAAATCGGTT1372882.242409849041429No Hit
GTAAACATCCTCGACTGGAA1206551.97073276860391No Hit
GAGGTAGTAGTTTGTACAGT1128811.843755216549484No Hit
ACCCGTAGATCCGATCTTGT1033421.6879488274258443No Hit
GCAGCATTGTACAGGGCTAT929971.5189775416009104No Hit
ACCCGTAGATCCGAACTTGT929661.5184711994200912No Hit
TCACAGTGGCTAAGTTCTGC822591.3435871436126894No Hit
TAAAGCTAGATAACCGAAAG814561.3304712477676026No Hit
GTAAACATCCCCGACTGGAA696151.1370648683134656No Hit
AGCTTATCAGACTGATGTTG676961.1057206539588935No Hit
CCCTGAGACCCTTTAACCTG671521.096835165366456No Hit
AAAGCTAGATAACCGAAAGT671031.0960348180483874No Hit
AAGGCACGCGGTGAATGCCA664671.085646636532229No Hit
TACAGTACTGTGATAACTGA647181.0570791373627935No Hit
CGAGGAGCTCACAGTCTAGT637671.0415458659447643No Hit
GTAAACATCCTACACTCTCA576930.9423354657417048No Hit
GTAAACATCCTTGACTGGAA491810.8033037030600382No Hit
TCAAGTAATTCAGGATAGGT474920.7757162210147686No Hit
GAATTGTGGCTGGACATCTG428140.6993075525673018No Hit
ACAGTACTGTGATAACTGAA413490.6753788011189182No Hit
GGAAGACTAGTGATTTTGTT405300.6620015673740539No Hit
ACATTCATTGCTGTCGGTGG355130.5800558021750499No Hit
AGCAGCACGTAAATATTGGC333060.5440075056244816No Hit
TCACATTGCCAGGGATTACC332880.5437135004872319No Hit
GAGGTAGTAGGTTGTGTGGT323430.5282782307816192No Hit
CGTACCGTGAGTAATAATGC321200.5246358338034693No Hit
TATAAAGCAATGAGACTGAT301890.49309561602406393No Hit
TAAGGCACGCGGTGAATGCC299290.4888488751526785No Hit
ATTGCACTTGTCCCGGCCTG290980.475275637982981No Hit
ACCCGTAGAACCGACCTTGC286170.4674191673709179No Hit
GAGGTAGTAGTTTGTGCTGT281120.45917068990918836No Hit
AACGGAATCCCAAAAGCAGC271280.44309840907286785No Hit
GGCTCAGTTCAGCAGGAACA269130.4395866810446068No Hit
TTTGGTTATCTAGCTGTATG265070.432955231837751No Hit
AACCGTTACCATTACTGAGT263560.43048885540860016No Hit
CCAGCATCAGTGATTTTGTT249980.408307801164979No Hit
GCTACATTGTCTGCTGGGTT249270.4071481142347161No Hit
GAGGTAGTAGGTTGTATGGT201210.3286487425890289No Hit
CCACAGGGTAGAACCACGGA188790.30836238811879507No Hit
TCACAGTGGCTAAGTTCTGA181400.2962918438728186No Hit
CTACAGTGCACGTGTCTCCA180200.29433180962448685No Hit
TGTGCTTGATCTAACCATGT169750.27726317804526435No Hit
ACCACAGGGTAGAACCACGG156690.2559314719759203No Hit
AAGGCACGCGGTGAATGCCC155380.2537917679214915No Hit
CTTTGGTTATCTAGCTGTAA153810.2512273897799241No Hit
ATTGCACTCGTCCCGGCCTC143970.2351551089436036No Hit
GTAAACATCCTACACTCAGC141780.23157804644039814No Hit
ACAATATCCTGGTGCTGAGT139800.22834398993065072No Hit
AGCACCATTTGAAATCGGTT135300.22099386149940659No Hit
CGGATCCGTCTGAGCTTGGC134880.2203078495124905No Hit
CCCTGAGACCCTAACTTGTC134810.22019351418133778No Hit
TCACATTGCCAGGGATTTCC134310.21937683324453292No Hit
CAAGAGCAATAACGAAAAAT132780.2168777895779099No Hit
GCTCGGTCTGAGGCCCCTCA130820.21367640030563467No Hit
GAGACCTCTGGGTTCTGAGC130470.21310472364987126No Hit
AGCTGCCAGTTGAAGAACTG130410.21300672193745465No Hit
TAGCACCATCTGAAATCGGT128450.20980533266517945No Hit
GAGGTAGTAAGTTGTATTGT126940.20733895623602866No Hit
ATCGTACAGGGTCATCCACT121630.19866580468716055No Hit
TCACAGTGGCTAAGTTCTGT118300.1932267096480399No Hit
GCTACATCTGGCTACTGGGT116850.1908583349313057No Hit
TAGACTGAAGCTCCTTGAGG116440.1901886565631257No Hit
CAGTGCATCACAGAACTTTG109370.17864078811670436No Hit
GAGGTAGTAGGTTGCATAGT108110.176582752155956No Hit
GAGATGAAGCACTGTAGCTT107590.17573340398167892No Hit
CCCTGAGACCCTAACTTGTT106050.1732180266963198No Hit
ATTATTACTTTTGGTACGCG103360.16882428325630944No Hit
TCACAGTGGCTAAGTTCTGG98050.16015113170744139No Hit
GAGGTAGGAGGTTGTATAGT95210.15551238398638953No Hit
TCTCACTACTGCACTTGACT92120.15046529579693524No Hit
TCAACGGGTATTTATTGAGC85870.14025678408687395No Hit
GAGATGAAGCACTGTAGCTA85070.1389500945879861No Hit
TATAATACAACCTGATAAGT82850.1353240312285723No Hit
TCCAGTTTTCCCAGGAATCC82100.13409900982336498No Hit
GAGAACTGAATTCCATAGGC80190.13097928864477026No Hit
GTACCGTGAGTAATAATGCG79240.12942759486484093No Hit
CCCTGAGACCCTAACTTGTA78050.12748389423524528No Hit
AAAGTGCTGTTCGTGCAGGT77570.12669988053591255No Hit
AAGGCACGCGGTGAATGCCT77440.12648754349234329No Hit
CAAGTAATCCAGGATAGGCT77430.1264712098736072No Hit
ATGGCTTTTTATTCCTATGT77070.12588319959910768No Hit
AAAGAATTCTCCTTTTGGGC76680.12524618846839985No Hit
TATCAGAATCTCCAGGGGTA72190.11791239365589182No Hit
AAAGCTGGGTTGAGAGGGCG70490.11513567847075513No Hit
ATTGCACTTGTCTCGGTCTG70180.1146293362899361No Hit
TCAAGTAATCCAGGATAGGA69350.11327364593483996No Hit
AGCACCATTTGAAATCAGTG65890.10762221385215004No Hit
CCTGAGACCCTAACTTGTGA65040.1062338562595817No Hit
CTCACACAGAAATCGCACCC64320.10505783571058264No Hit
CTGGCTCCGTGTCTTCACTC63230.10327747126834796No Hit
AAGGCACGCGGTGAATGCCG62740.10247712395027916No Hit
ACAGTACTGTGATAACTGAC62230.10164410939473817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)