FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004956038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004956038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5526517
Sequences flagged as poor quality0
Sequence length18-20
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTTATCTAGCTGTAT70761812.804050001112815No Hit
TCAAGTAATCCAGGATAGGC3191795.775409720082287No Hit
CCCTGAGACCCTAACTTGTG1700143.076331801747828No Hit
AGCACCATCTGAAATCGGTT1541532.789333679784211No Hit
GAGGTAGTAGATTGTATAGT1296012.345075569296177No Hit
GTAAACATCCTCGACTGGAA1283572.3225659126715796No Hit
GAGATGAAGCACTGTAGCTC1249062.26012151957553No Hit
AAGGCACGCGGTGAATGCCA1134012.051943384956565No Hit
ACCCGTAGATCCGATCTTGT1047461.8953348012862352No Hit
GCAGCATTGTACAGGGCTAT1017501.841123441762687No Hit
GAGGTAGTAGGTTGTATAGT1016191.838753051876978No Hit
TAAAGCTAGATAACCGAAAG981871.7766524557872525No Hit
GTAAACATCCCCGACTGGAA827771.4978149890790167No Hit
GAGGTAGTAGTTTGTACAGT818531.4810955978240907No Hit
TACAGTACTGTGATAACTGA745341.3486613720721388No Hit
ACATTCAACGCTGTCGGTGA702401.2709632486428613No Hit
GTAAACATCCTTGACTGGAA672971.2177109018211651No Hit
AAAGCTAGATAACCGAAAGT602811.0907593335911208No Hit
TAAGGCACGCGGTGAATGCC592301.0717419307676064No Hit
GTAAACATCCTACACTCTCA549700.9946590230338566No Hit
GGAAGACTAGTGATTTTGTT525210.9503453983765906No Hit
ACAGTACTGTGATAACTGAA410120.7420948854405044No Hit
ACCCGTAGATCCGAACTTGT407510.7373722002483661No Hit
CCCTGAGACCCTTTAACCTG403800.7306591113354035No Hit
CGTACCGTGAGTAATAATGC394070.7130530856957465No Hit
AGCTTATCAGACTGATGTTG370610.6706032027043434No Hit
TTTGGTTATCTAGCTGTATG361180.6535400144430932No Hit
TGTGCTTGATCTAACCATGT347960.6296189806346384No Hit
AAGGCACGCGGTGAATGCCC314860.5697259232171005No Hit
ACCCGTAGAACCGACCTTGC286160.5177944806828605No Hit
TCAAGTAATTCAGGATAGGT285600.516781184243168No Hit
AGCTGCCAGTTGAAGAACTG268540.4859118319911076No Hit
CGAGGAGCTCACAGTCTAGT260530.471418073987649No Hit
GCTACATTGTCTGCTGGGTT258700.4681067659793682No Hit
AACGGAATCCCAAAAGCAGC257320.46560971403869744No Hit
AGCAGCACGTAAATATTGGC247630.44807606671616135No Hit
AACCGTTACCATTACTGAGT241500.43698408961738466No Hit
CAAGAGCAATAACGAAAAAT238730.431971891156763No Hit
GAGGTAGTAGTTTGTGCTGT229930.4160486613901667No Hit
GAGGTAGTAGGTTGTGTGGT228860.4141125414071829No Hit
GGCTCAGTTCAGCAGGAACA221540.4008673093740597No Hit
TCACATTGCCAGGGATTACC220030.39813502790274596No Hit
GAGACCTCTGGGTTCTGAGC207920.376222492394396No Hit
CGGATCCGTCTGAGCTTGGC197860.35801934563849164No Hit
TATAAAGCAATGAGACTGAT196080.354798510526612No Hit
GAGGTAGTAGGTTGTATGGT191610.3467102335883523No Hit
AGCACCATTTGAAATCGGTT190130.3440322358548793No Hit
TAGCACCATCTGAAATCGGT189940.3436884388485551No Hit
AAGGCACGCGGTGAATGCCT189580.34303703399446706No Hit
GTAAACATCCTACACTCAGC187930.3400514284132303No Hit
ATCGTACAGGGTCATCCACT183090.3312936520416023No Hit
GGTAGACTATGGAACGTAGG162750.29448927778562883No Hit
CTTTGGTTATCTAGCTGTAA143610.259856253043282No Hit
GAGAACTGAATTCCATAGGC143450.2595667397747985No Hit
TCAACGGGTATTTATTGAGC140470.25417455514929205No Hit
AAGGCACGCGGTGAATGCCG138040.24977757238419787No Hit
ATTATTACTTTTGGTACGCG134900.24409587449020784No Hit
ACATTCATTGCTGTCGGTGG131090.2372018397844429No Hit
GAGGTAGTAAGTTGTATTGT126510.22891452247410077No Hit
CCACAGGGTAGAACCACGGA124390.2250784716666935No Hit
GCTACATCTGGCTACTGGGT117760.2130817656039057No Hit
TTTTTGCGGTCTGGGCTTGC117010.21172467215788895No Hit
CAGTGCATCACAGAACTTTG114920.20794290508832236No Hit
GAATTGTGGCTGGACATCTG113720.20577155557469562No Hit
ATTGCACTTGTCCCGGCCTG111460.20168218065736523No Hit
ACCACAGGGTAGAACCACGG109000.19723091415443034No Hit
ATGGCTTTTTATTCCTATGT108780.19683283341026545No Hit
AGCACCATTTGAAATCAGTG107210.19399198446327046No Hit
GTACCGTGAGTAATAATGCG103880.1879664895629562No Hit
GCTCGGTCTGAGGCCCCTCA93030.16833387104391428No Hit
GAGATGAAGCACTGTAGCTT91980.16643394021949087No Hit
CCAGCATCAGTGATTTTGTT90850.16438925276082567No Hit
ATTGCACTCGTCCCGGCCTC90710.16413592865090254No Hit
CTCACACAGAAATCGCACCC86410.15635525956040666No Hit
TATAATACAACCTGATAAGT82800.14982311644024618No Hit
GCTGGTGTTGTGAATCAGGC82370.14904504953119657No Hit
TATTGCTTAAGAATACGCGT76290.13804354532882104No Hit
CCCTGAGACCCTAACTTGTC76000.13751880252969456No Hit
ATGACACGATCACTCCCGTT75840.13722928926121097No Hit
AAAGAATTCTCCTTTTGGGC75420.13646931693144163No Hit
TCACATTGCCAGGGATTTCC74460.13473223732054024No Hit
GAGGTAGTAGGTTGCATAGT71520.12941243101215466No Hit
CTGGCTCCGTGTCTTCACTC71320.1290505394265502No Hit
ACATTCAACCTGTCGGTGAG70790.1280915267246984No Hit
GAGGTAGGAGGTTGTATAGT70300.12720489233996746No Hit
ATAATACATGGTTGATCTTT69820.12633635253451678No Hit
TAGACTGAAGCTCCTTGAGG68140.12329646321543931No Hit
AGAAAGCTCACAAGAACTGC67170.12154128902525768No Hit
TAGTAGACCGTATAGCGTAC63310.11455678142309161No Hit
CCCTGAGACCCTAACTTGTT60460.10939982632822806No Hit
CAAGTAATCCAGGATAGGCT60450.10938173174894784No Hit
TTGGTTATCTAGCTGTATGA59960.10849509736421692No Hit
ATATAACACAGATGGCCTGT59100.10693896354611775No Hit
AGCAGCACAGAAATATTGGC58670.10616089663706815No Hit
AACAAACATGGTGCACTTCT57890.10474951945321077No Hit
TAAGGCACGCGGTGAATGCT55580.10056967163947926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)