FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004956039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004956039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847319
Sequences flagged as poor quality0
Sequence length18-20
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTTATCTAGCTGTAT3829559.95381459140768No Hit
TCAAGTAATCCAGGATAGGC1568424.076657017523112No Hit
CCCTGAGACCCTAACTTGTG1020582.6527043897321745No Hit
GAGATGAAGCACTGTAGCTC852502.215828736842461No Hit
AGCACCATCTGAAATCGGTT767211.9941418946544336No Hit
GTAAACATCCTCGACTGGAA724031.8819078948223427No Hit
TAAAGCTAGATAACCGAAAG604661.5716398874125075No Hit
GCAGCATTGTACAGGGCTAT570141.4819150686491034No Hit
GAGGTAGTAGATTGTATAGT554371.440925486033261No Hit
ACATTCAACGCTGTCGGTGA544221.4145434781987145No Hit
GAGGTAGTAGGTTGTATAGT514781.3380226594155566No Hit
GTAAACATCCCCGACTGGAA498271.2951096594797573No Hit
ACCCGTAGATCCGATCTTGT489791.2730683366780868No Hit
AAGGCACGCGGTGAATGCCA478801.2445029902641294No Hit
GAGGTAGTAGTTTGTACAGT401591.0438177858399578No Hit
AAAGCTAGATAACCGAAAGT383660.9972139040199162No Hit
GGAAGACTAGTGATTTTGTT371840.9664912111524935No Hit
TACAGTACTGTGATAACTGA358890.9328314080532444No Hit
GTAAACATCCTTGACTGGAA344290.8948829041730099No Hit
TCTCACTACTGCACTTGACT324330.843002620786059No Hit
CCCTGAGACCCTTTAACCTG311100.8086150381603396No Hit
TAAGGCACGCGGTGAATGCC276610.718968195774772No Hit
ACCCGTAGATCCGAACTTGT269500.7004877942276166No Hit
GTAAACATCCTACACTCTCA266150.691780432035919No Hit
CCCCACTGCTAAATTTGACT263720.685464345431195No Hit
AGCTTATCAGACTGATGTTG231420.6015097786276625No Hit
CGAGGAGCTCACAGTCTAGT214410.5572971723945948No Hit
CCACTGCTAAATTTGACTGG202840.5272242826758062No Hit
CGTACCGTGAGTAATAATGC201460.5236373692953457No Hit
TTTGGTTATCTAGCTGTATG201280.5231695110283291No Hit
AAGGCACGCGGTGAATGCCC172800.44914393633592636No Hit
CCCACTGCTAAATTTGACTG167850.4362778339929702No Hit
CCCCCACTGCTAAATTTGAC166700.43328873950925306No Hit
TCACATTGCCAGGGATTACC164530.4276484481791086No Hit
TCAAGTAATTCAGGATAGGT158630.41231309387134263No Hit
GGCTCAGTTCAGCAGGAACA158230.411273408833528No Hit
TATAAAGCAATGAGACTGAT151580.3939886450798595No Hit
ACAGTACTGTGATAACTGAA148900.38702275532650143No Hit
ACCCGTAGAACCGACCTTGC145050.37701578683753545No Hit
AGCAGCACGTAAATATTGGC132910.345461345939861No Hit
TGTGCTTGATCTAACCATGT132500.344395668776101No Hit
GCTACATTGTCTGCTGGGTT131530.3418744325594004No Hit
AACGGAATCCCAAAAGCAGC130280.3386254168162297No Hit
TTCTCACTACTGCACTTGAC126770.32950218060940617No Hit
GAATTGTGGCTGGACATCTG125890.327214873526214No Hit
AGCTGCCAGTTGAAGAACTG125860.32713689714837785No Hit
GAGGTAGTAGTTTGTGCTGT118760.30868248772716794No Hit
AACCGTTACCATTACTGAGT117790.3061612515104674No Hit
CCCACTGCTTCACTTGACTA107440.2792594011570135No Hit
GAGACCTCTGGGTTCTGAGC105830.2750746688798095No Hit
CGGATCCGTCTGAGCTTGGC105390.2739310153382134No Hit
ACATTCATTGCTGTCGGTGG105210.27346315707119684No Hit
GAGGTAGTAGGTTGTGTGGT100160.26033713346878695No Hit
CAAGAGCAATAACGAAAAAT96380.25051210986143857No Hit
GCTACATCTGGCTACTGGGT94660.2460414641988356No Hit
ATCGTACAGGGTCATCCACT93350.24263649569999265No Hit
TAGCACCATCTGAAATCGGT87620.22774300753329785No Hit
AGCACCATTTGAAATCGGTT84080.21854179494863826No Hit
GAGAACTGAATTCCATAGGC83920.2181259209335124No Hit
CTTTGGTTATCTAGCTGTAA78920.20512985796082936No Hit
AAGGCACGCGGTGAATGCCT77420.20123103906902443No Hit
CCAGCATCAGTGATTTTGTT75210.1954867792350985No Hit
GTAAACATCCTACACTCAGC74420.19343340128541459No Hit
CCACAGGGTAGAACCACGGA72530.1885208894817404No Hit
GAGGTAGTAGGTTGTATGGT70030.18202285799539888No Hit
AAGGCACGCGGTGAATGCCG69330.18020340917922326No Hit
ATTGCACTTGTCCCGGCCTG69160.179761543038152No Hit
TCAACGGGTATTTATTGAGC67300.17492700761231392No Hit
CCCTGAGACCCTAACTTGTC64140.16671349581357822No Hit
TCACATTGCCAGGGATTTCC63450.164920039123348No Hit
CTCACTACTGCACTTGACTA63390.16476408636767578No Hit
AACAGTCTACAGCCATGGTC63250.16440019660444066No Hit
ACCACAGGGTAGAACCACGG62020.16120316511316063No Hit
GAGATGAAGCACTGTAGCTT61180.15901982653374985No Hit
ATGGCTTTTTATTCCTATGT59760.1553289446495079No Hit
CAGTGCATCACAGAACTTTG59360.15428925961169324No Hit
ATTATTACTTTTGGTACGCG59290.15410731473007566No Hit
GCTCGGTCTGAGGCCCCTCA56890.1478692045031878No Hit
GTACCGTGAGTAATAATGCG54780.14238486592871555No Hit
CCCTGAGACCCTAACTTGTT54520.14170907065413604No Hit
GAGGTAGTAAGTTGTATTGT54270.14105926750550188No Hit
GGTAGACTATGGAACGTAGG53450.13892791317798187No Hit
TTTTTGCGGTCTGGGCTTGC53240.13838207853312917No Hit
TAGACTGAAGCTCCTTGAGG43320.11259788959532599No Hit
ATGACACGATCACTCCCGTT41980.10911494471864693No Hit
ACAATATCCTGGTGCTGAGT40440.10511215732306055No Hit
TATAATACAACCTGATAAGT40390.10498219669333372No Hit
CTCACACAGAAATCGCACCC39220.1019411179577259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)