FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004956040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004956040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365790
Sequences flagged as poor quality0
Sequence length18-20
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTTATCTAGCTGTAT69634912.977567142955651No Hit
TCAAGTAATCCAGGATAGGC3232936.025077388418108No Hit
CCCTGAGACCCTAACTTGTG2093143.9008980970183327No Hit
AGCACCATCTGAAATCGGTT1478692.7557731480359835No Hit
GAGATGAAGCACTGTAGCTC1423862.653588753939308No Hit
GTAAACATCCTCGACTGGAA1252602.334418603784345No Hit
GCAGCATTGTACAGGGCTAT1113652.0754632589050264No Hit
GAGGTAGTAGATTGTATAGT1027001.9139772521846736No Hit
TAAAGCTAGATAACCGAAAG1015361.8922842675542653No Hit
GTAAACATCCCCGACTGGAA998951.8617016320057251No Hit
AAGGCACGCGGTGAATGCCA972891.8131346921888483No Hit
ACCCGTAGATCCGATCTTGT934151.740936562929224No Hit
GAGGTAGTAGGTTGTATAGT890021.6586933144979583No Hit
GAGGTAGTAGTTTGTACAGT763381.422679605426228No Hit
AAAGCTAGATAACCGAAAGT661821.2334064508674398No Hit
ACATTCAACGCTGTCGGTGA658921.2280018412945717No Hit
CCCTGAGACCCTTTAACCTG631221.1763785015813142No Hit
GTAAACATCCTACACTCTCA618671.152989587740109No Hit
TACAGTACTGTGATAACTGA596721.1120822842489175No Hit
GGAAGACTAGTGATTTTGTT559421.0425678231909934No Hit
GTAAACATCCTTGACTGGAA542521.011071994990486No Hit
TAAGGCACGCGGTGAATGCC524440.9773770497913635No Hit
CGTACCGTGAGTAATAATGC356910.6651583457421927No Hit
TTTGGTTATCTAGCTGTATG356780.6649160701406502No Hit
ACCCGTAGATCCGAACTTGT348140.6488140609304501No Hit
AGCTTATCAGACTGATGTTG319680.5957743407774065No Hit
ACAGTACTGTGATAACTGAA314840.5867542337661369No Hit
AAGGCACGCGGTGAATGCCC309400.5766159316708258No Hit
TGTGCTTGATCTAACCATGT303860.5662912637281742No Hit
AACGGAATCCCAAAAGCAGC281430.5244894041697494No Hit
CGAGGAGCTCACAGTCTAGT278580.5191779775205515No Hit
ACCCGTAGAACCGACCTTGC267530.49858455138945057No Hit
TCACATTGCCAGGGATTACC263340.49077582238589285No Hit
GCTACATTGTCTGCTGGGTT261720.4877566956589803No Hit
TCAAGTAATTCAGGATAGGT251590.46887783532340993No Hit
GGCTCAGTTCAGCAGGAACA233640.43542516572582973No Hit
GAGGTAGTAGTTTGTGCTGT224810.4189690614056831No Hit
AGCAGCACGTAAATATTGGC219070.40827166176835095No Hit
TATAAAGCAATGAGACTGAT212790.3965678865553814No Hit
CAAGAGCAATAACGAAAAAT210880.39300829887118205No Hit
CGGATCCGTCTGAGCTTGGC203600.3794408651848097No Hit
GCTACATCTGGCTACTGGGT191300.3565178659619553No Hit
AGCTGCCAGTTGAAGAACTG187970.350311883245524No Hit
GAGGTAGTAGGTTGTGTGGT185450.34561546389254894No Hit
GAGAACTGAATTCCATAGGC179750.334992610594153No Hit
ATCGTACAGGGTCATCCACT171550.31971061111225No Hit
GAGACCTCTGGGTTCTGAGC169760.316374662444859No Hit
TAGCACCATCTGAAATCGGT162550.30293768485162487No Hit
GTAAACATCCTACACTCAGC159990.29816671915971366No Hit
GAGGTAGTAGGTTGTATGGT153610.2862765780994038No Hit
AGCACCATTTGAAATCGGTT151500.28234425872052393No Hit
AACCGTTACCATTACTGAGT148360.2764923711140391No Hit
CTTTGGTTATCTAGCTGTAA144900.27004411279606544No Hit
AAGGCACGCGGTGAATGCCT144900.27004411279606544No Hit
AAGGCACGCGGTGAATGCCG130610.24341243321113945No Hit
TTTTTGCGGTCTGGGCTTGC125710.23428050669146572No Hit
CCACAGGGTAGAACCACGGA122850.22895044345753374No Hit
TCAACGGGTATTTATTGAGC122640.22855907517811916No Hit
ACATTCATTGCTGTCGGTGG121380.22621086550163164No Hit
CCCTGAGACCCTAACTTGTC114410.21322116594201415No Hit
ATTGCACTTGTCCCGGCCTG109940.20489061256590363No Hit
ATGGCTTTTTATTCCTATGT108970.2030828638467029No Hit
GAGGTAGTAAGTTGTATTGT104470.19469640071639033No Hit
GGTAGACTATGGAACGTAGG101090.18839723507628886No Hit
ATTATTACTTTTGGTACGCG101040.18830405215261872No Hit
ACCACAGGGTAGAACCACGG100090.18653357660288605No Hit
GTACCGTGAGTAATAATGCG97630.18194897675831517No Hit
CCCTGAGACCCTAACTTGTT97090.18094260118267766No Hit
GAGATGAAGCACTGTAGCTT95030.1771034647274679No Hit
CAGTGCATCACAGAACTTTG94420.1759666330586922No Hit
GCTCGGTCTGAGGCCCCTCA88920.16571651145497682No Hit
CTCACACAGAAATCGCACCC88290.16454240661673306No Hit
TCACATTGCCAGGGATTTCC87840.1637037603037018No Hit
GAATTGTGGCTGGACATCTG87020.16217556035551148No Hit
CTGGCTCCGTGTCTTCACTC85160.1587091555949823No Hit
ATTGCACTCGTCCCGGCCTC84730.15790778245141907No Hit
GCTGGTGTTGTGAATCAGGC80270.1495958656600426No Hit
ATGACACGATCACTCCCGTT79560.14827266814392662No Hit
AACAGTCTACAGCCATGGTC73540.13705344413404177No Hit
AACAAACATGGTGCACTTCT71540.13332612718723616No Hit
ATAATACATGGTTGATCTTT67960.12665422985245414No Hit
CCAGCATCAGTGATTTTGTT67160.1251633030737319No Hit
GAGGTAGGAGGTTGTATAGT66840.12456693236224303No Hit
TCTCACTACTGCACTTGACT65990.12298282265985065No Hit
TAAGGCACGCGGTGAATGCT65510.12208826659261732No Hit
AGCACCATTTGAAATCAGTG65220.12154780563533049No Hit
TATAATACAACCTGATAAGT63160.11770866918012071No Hit
GAGGTAGTAGGTTGCATAGT62530.11653456434187695No Hit
TATTGCTTAAGAATACGCGT62450.11638547166400474No Hit
AAGGCACGCGGTGAATGCTT61990.11552818876623945No Hit
TTGGTTATCTAGCTGTATGA61880.11532318633416515No Hit
GAGATGAAGCACTGTAGCTA61430.1144845400211339No Hit
AGAAAGCTCACAAGAACTGC61190.11403726198751722No Hit
CAAGTAATCCAGGATAGGCT60820.11334770835235819No Hit
AAGGCACGCGGTGAATGCTC59470.1108317694132644No Hit
TATCAGAATCTCCAGGGGTA59400.11070131332012621No Hit
CCCTGAGACCCTAACTTGTA58930.10982539383762688No Hit
GGAATGTAAAGAAGTATGTA56430.10516624765411989No Hit
ACACACCTGGTTAACCTCTT55820.10402941598534418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)