FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004956041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004956041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5039957
Sequences flagged as poor quality0
Sequence length18-20
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTTATCTAGCTGTAT55899111.091185897022534No Hit
TCAAGTAATCCAGGATAGGC3613067.168831003915312No Hit
GAGGTAGTAGGTTGTATAGT1909653.7890204221980466No Hit
GAGGTAGTAGATTGTATAGT1701523.376060549722944No Hit
CCCTGAGACCCTAACTTGTG1371132.7205192425252838No Hit
CACAGTGAACCGGTCTCTTT1355012.68853484265838No Hit
ACATTCAACGCTGTCGGTGA1104152.1907925008090348No Hit
AGCACCATCTGAAATCGGTT1043532.070513696843048No Hit
GAGGTAGTAGTTTGTACAGT1034752.053092913292713No Hit
GTAAACATCCTCGACTGGAA1003101.9902947584671853No Hit
AAGGCACGCGGTGAATGCCA876571.7392410292389398No Hit
GCAGCATTGTACAGGGCTAT817321.6216805024328582No Hit
ACCCGTAGATCCGATCTTGT785411.5583664701901228No Hit
TAAAGCTAGATAACCGAAAG753561.4951714865821275No Hit
GAGATGAAGCACTGTAGCTC750301.4887031774279027No Hit
GTAAACATCCCCGACTGGAA525241.0421517485169018No Hit
AAAGCTAGATAACCGAAAGT524871.0414176152693366No Hit
TCAAGTAATTCAGGATAGGT461900.9164760731093539No Hit
AGCTTATCAGACTGATGTTG456180.9051267699307752No Hit
GGAAGACTAGTGATTTTGTT430660.8544914172878856No Hit
TCACAGTGGCTAAGTTCTGC421320.83595951314664No Hit
AACCGTTACCATTACTGAGT409770.8130426509591252No Hit
TAAGGCACGCGGTGAATGCC402670.7989552291815188No Hit
ACCCGTAGATCCGAACTTGT398510.7907011905061888No Hit
CCCTGAGACCCTTTAACCTG392310.7783994982496875No Hit
GTAAACATCCTTGACTGGAA385260.764411283667698No Hit
TACAGTACTGTGATAACTGA373620.7413158485280728No Hit
GTAAACATCCTACACTCTCA336830.6683191939931233No Hit
CGTACCGTGAGTAATAATGC319030.6330014323534903No Hit
GAGGTAGTAGTTTGTGCTGT318720.6323863477406653No Hit
GAGGTAGTAGGTTGTGTGGT314020.6230608713526722No Hit
GAGGTAGTAGGTTGTATGGT278490.5525642381472698No Hit
AGCAGCACGTAAATATTGGC277030.5496673880352551No Hit
GCTACATTGTCTGCTGGGTT262400.5206393625977365No Hit
TTTGGTTATCTAGCTGTATG252870.5017304711131464No Hit
CGAGGAGCTCACAGTCTAGT245960.4880200366788844No Hit
GGCTCAGTTCAGCAGGAACA227150.450698289687789No Hit
ACAGTACTGTGATAACTGAA226630.44966653485337277No Hit
AACGGAATCCCAAAAGCAGC205710.4081582442072422No Hit
TCACATTGCCAGGGATTACC204390.4055391742429549No Hit
ACATTCATTGCTGTCGGTGG200020.3968684653460337No Hit
AAGGCACGCGGTGAATGCCC186940.37091586297264045No Hit
ACCCGTAGAACCGACCTTGC180080.35730463573399535No Hit
GAGGTAGTAAGTTGTATTGT169820.3369473191933979No Hit
TGTGCTTGATCTAACCATGT168930.33518143111141624No Hit
CTACAGTGCACGTGTCTCCA156840.3111931312112385No Hit
AGCACCATTTGAAATCGGTT136490.2708158025951412No Hit
AAGGCACGCGGTGAATGCCT134980.2678197452875094No Hit
ATTGCACTTGTCCCGGCCTG133100.2640895547323122No Hit
GAATTGTGGCTGGACATCTG130880.25968475524691975No Hit
CCAGCATCAGTGATTTTGTT128170.25430772524448125No Hit
CTTTGGTTATCTAGCTGTAA124220.24647035679074247No Hit
AGCTGCCAGTTGAAGAACTG122940.24393065258294863No Hit
CCACAGGGTAGAACCACGGA118230.23458533475583226No Hit
CAAGAGCAATAACGAAAAAT117890.233910725825637No Hit
GCTACATCTGGCTACTGGGT115860.22988291368358896No Hit
ATCGTACAGGGTCATCCACT114900.22797813552774357No Hit
TATAAAGCAATGAGACTGAT114720.22762098962352256No Hit
GTAAACATCCTACACTCAGC114110.22641066183699582No Hit
GAGGTAGTAGGTTGCATAGT111060.2203590229043621No Hit
CGGATCCGTCTGAGCTTGGC103190.20474381031425465No Hit
ATGGCTTTTTATTCCTATGT99840.19809692820791924No Hit
ATTATTACTTTTGGTACGCG97260.1929778369140848No Hit
ACCACAGGGTAGAACCACGG97200.19285878827934444No Hit
TCACATTGCCAGGGATTTCC96290.191053217319116No Hit
GCTCGGTCTGAGGCCCCTCA94770.18803731857236083No Hit
TCAACGGGTATTTATTGAGC93700.1859142845861582No Hit
ATTGCACTCGTCCCGGCCTC92570.18367220196521516No Hit
GAGACCTCTGGGTTCTGAGC89190.17696579554150957No Hit
GAGAACTGAATTCCATAGGC86710.172045118638909No Hit
AGCACCATTTGAAATCAGTG85820.17027923055692737No Hit
AAGGCACGCGGTGAATGCCG83600.16587443107153493No Hit
GAGGTAGGAGGTTGTATAGT81140.16099343704718116No Hit
TAGCACCATCTGAAATCGGT80870.16045771819084964No Hit
CAAGTAATCCAGGATAGGCT79360.15746166088321786No Hit
TCACAGTGGCTAAGTTCTGA78990.15672752763565245No Hit
GTACCGTGAGTAATAATGCG76850.15248145966324714No Hit
GAGATGAAGCACTGTAGCTT74700.1482155502517184No Hit
AGCAGCACAGAAATATTGGC71610.14208454556259112No Hit
TATTGCTTAAGAATACGCGT68840.13658846692541227No Hit
GCTGGTGTTGTGAATCAGGC67540.13400907983937166No Hit
AAAGTGCTGTTCGTGCAGGT67270.13347336098304013No Hit
GGTAGACTATGGAACGTAGG66210.13137016843596086No Hit
CCCTGAGACCCTAACTTGTC65850.13065587662751885No Hit
TCTCACTACTGCACTTGACT64230.12744156348952976No Hit
CTCACACAGAAATCGCACCC59710.11847323300575778No Hit
TCACAGTGGCTAAGTTCTGT58020.115120029793905No Hit
ACAATATCCTGGTGCTGAGT55600.11031840152604477No Hit
AAAGAATTCTCCTTTTGGGC55340.10980252410883663No Hit
TATCAGAATCTCCAGGGGTA55230.10958426827847936No Hit
AAAGCTGGGTTGAGAGGGCG55140.10940569532636886No Hit
CAGTGCATCACAGAACTTTG54840.10881045215266717No Hit
CTGGCTCCGTGTCTTCACTC54740.10861203776143329No Hit
TTTTTGCGGTCTGGGCTTGC54360.10785806307474449No Hit
AACAAACATGGTGCACTTCT51190.10156832687263007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)