FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004956043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004956043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21218217
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24350.020.9929941
CGTATCG19050.020.0749023
GTCCGAT20950.016.4326341
CCGTATC28650.016.2606892
ATCGCGC24150.014.34030367
CCGATTG21700.014.0986863
TCCGATT39900.013.2443142
CATCGCG27450.013.19912666
GTCCGTT23000.012.5489181
TCGACGT22150.012.3997272
TCGCGCC28600.012.33998368
TACATCG28950.012.01957364
CGCGCCA31700.011.8969569
CCCGTCT66050.011.8458361
CTCGTAT19700.011.8267271
GTCGGAT15500.011.4418031
GCGCCAT46100.011.36466270
ATCGAAG44200.011.0903546
TATCGAG24250.011.0388397
CGTGTTA30750.010.97226459
Produced by FastQC (version 0.11.5)