FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004961627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17777497
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT3754232.11178772804743TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA1638240.92152455432843TruSeq Adapter, Index 14 (97% over 44bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA693830.39028553907223273No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA273740.15398118194029228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)