FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18799444
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC266480.14174887299858444TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC38800.034.01967644
TCGTATG42250.031.18942343
CTCGTAT43800.029.93349342
TATCTCG45850.028.11654939
GATCGGA49200.027.1487331
CGCCAAT49600.026.69995133
AGTCACG52600.025.30287728
TTGAACG32800.024.3459177
TCGAACG30850.024.24529640
ACACGTC55550.024.15652813
GTCACGC55000.024.11875529
ATCTCGT55800.023.33948340
ATCGGAA59650.022.5769882
ACGTCTG59900.022.3655315
TCGGAAG62400.021.7190253
ACGCCAA62400.021.5755832
TCTCGTA61100.021.27894241
AACGGTA36450.020.88265843
CACGTCT64600.020.87452914
ACGGTAA36300.020.78730444
Produced by FastQC (version 0.11.5)