FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36191671
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT45950.029.20644244
TCGAACG40800.021.1887340
AGATCGG74900.020.664961
TTGAACG49500.019.2428877
ACGGTAA46100.018.7076644
CGAACGG47250.018.3894141
AACGGTA48150.018.04545843
GTCGAAC51600.016.96703339
GAACGGT53650.016.44151342
AGTCGAA55100.016.288538
GCCTAAC54600.016.15783124
CCGATCT93200.016.09685540
AACGCTG57500.015.99369210
GATCGGA97950.015.9786312
GAACGCT57200.015.9239679
TCCCGAT98100.015.49464338
TCTCGTA99850.015.40151444
CCCGATC97800.015.33974139
ATCTCGT102100.015.14595743
CGTCCCG105600.014.76910736
Produced by FastQC (version 0.11.5)