FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004961636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17539996
Sequences flagged as poor quality0
Sequence length57
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTC6781673.8664033902858357TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCT1683120.959589728526734TruSeq Adapter, Index 20 (97% over 44bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAG263940.15047893967592693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)