FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40849655
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA434770.10643174342598487TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT430850.1054721269983798TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT68600.031.49400144
TCGAACG46900.022.60738640
AGATCGG91700.022.5512581
ACGGTAA51000.020.62045344
TTGAACG55450.020.2726677
CGAACGG52850.019.97893741
AACGGTA54500.019.41417143
AGTCGAA61400.017.48346938
AACGATC120600.017.4193638
GTCGAAC61550.017.29790339
GAACGCT63000.017.2867839
GAACGGT62700.016.94530342
GAAACGA133150.016.53740736
ACGATCT128400.016.42970339
GATCGGA133750.016.2360251
AACGCTG68850.016.23305510
CGTGAAA135450.016.19139733
AAACGAT132700.016.11275737
ATCTCGT131200.016.06206742
GCCTAAC66850.015.99429924
Produced by FastQC (version 0.11.5)