FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18352793
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC249170.13576680127106538TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC42750.029.28081144
TCGTATG47550.026.41747743
CTCGTAT47800.025.91020842
TCGAACG28900.025.57657840
GATCGGA51650.024.836741
TTGAACG32450.024.0660067
ACACGTC56850.022.55938713
ACGGTAA32700.022.5374144
GCCTAAC34400.021.74434724
AACGGTA33800.021.73879843
GAACGCT36100.021.4497539
CGAACGG34350.021.39049141
AACGCTG36600.021.03651410
ATCTCGT58450.020.85097140
GTCGAAC35800.020.83136439
AGTCGAA35700.020.82809438
ATCGGAA61600.020.7911362
ACGTCTG61700.020.7860815
GAACGGT36000.020.7762342
CGTCTGA63350.020.3489216
Produced by FastQC (version 0.11.5)