FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45795051
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1765040.3854215600720698TruSeq Adapter, Index 22 (97% over 40bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA1233630.26938063678540286No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA1083440.23658451652341211TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT206000.039.43665744
AGATCGG166300.031.264871
TTGAACG196000.030.3908717
TCGACTA70600.029.10249544
AACGCTG206500.028.55868510
GAACGCT214600.027.5927499
TCGAACG216350.026.95471240
ACGTACG48750.026.71254226
ATTGAAC231150.026.4546156
ATACGAG54150.025.9978716
CATACGA54250.025.94994715
TTACGTA49600.025.89994224
ACGGTAA225150.025.8624744
GTCGAAC226100.025.84858739
GAACGGT226900.025.69208742
AGTCGAA228850.025.4996438
CGTACGT325150.025.22888434
TACGTAA327950.025.01970136
CGGCATA57000.025.00659212
CAGATTG248550.024.9936033
Produced by FastQC (version 0.11.5)