FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35725660
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT797690.223282089120257TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA742870.20793737610445825TruSeq Adapter, Index 19 (97% over 40bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA465100.13018653819131684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT99600.036.8976944
AGATCGG125650.028.841681
TTGAACG75800.027.3690977
TCGAACG81650.024.59844440
GAACGCT87150.023.2739759
ACGGTAA86200.023.2569744
AACGCTG88200.022.87219210
TCGACTA32000.022.48742744
GTCGAAC91900.021.97456239
AACGGTA91550.021.84231243
TATGCCG32100.021.6603623
CATACGA22850.021.46890415
CGAACGG94450.021.21824641
GAACGGT95200.021.07419442
CGTATGC33500.021.0437091
AGTCGAA96100.021.01417738
TGAACGC98150.020.912668
GCCTAAC96600.020.79145624
ATTGAAC109750.020.5063846
GCATACG25250.019.77678714
Produced by FastQC (version 0.11.5)