FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24353375
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG39500.025.84167340
TTGAACG42150.024.7907417
ACGGTAA45350.022.65417544
CGAACGG45600.022.5295141
CGTATGC22850.022.52881444
AACGGTA46600.021.999243
AGTCGAA47100.021.85871938
GAACGGT47100.021.85804642
GAACGCT48550.021.4317829
GTCGAAC48800.021.23248539
GCCTAAC50650.020.45818324
AACGCTG52150.020.07885710
AAGTCGA56550.018.5560537
TGAACGC56350.018.5042218
CAAGTCG58600.017.83182136
ATTGAAC65900.017.8258136
TCGACTA17800.017.79717844
GGCCTAA61250.017.09725823
TCGTATG31100.016.90620644
GATTGAA81700.015.5362885
Produced by FastQC (version 0.11.5)