FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34393522
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC661590.19235889828322902TruSeq Adapter, Index 5 (100% over 50bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA388910.11307652644588129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC97050.032.9011244
TCGTATG110550.028.97187443
CTCGTAT113250.027.912142
GATCGGA122650.026.307821
TTGAACG69100.025.4993487
ACACGTC130350.024.6581513
TCGAACG68550.024.22773440
ACGTCTG139100.023.26520315
CGTCTGA143350.022.60350816
ATCTCGT138000.022.60322840
ATCGGAA144300.022.2687262
CACGTCT147200.022.0746614
AACGCTG78600.021.80400310
ACGGTAA77100.021.72045944
TCGGAAG149750.021.4628623
GATCTCG147300.021.23587439
CACACGT152650.021.21447612
GAACGCT81150.021.1732529
TCTCGTA148350.021.01146141
GCCTAAC81200.020.9694324
Produced by FastQC (version 0.11.5)