FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20240896
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1891840.9346621809627399TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA446690.22068687077884297TruSeq Adapter, Index 18 (97% over 40bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA279810.13823992771861482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT213750.041.43594444
GATCGGA273550.032.9638441
CGCACAT266300.032.62240237
AGTCACG275000.032.39830428
ACGTCCG275850.032.33794832
ACACGTC277750.032.29074513
GTCACGT276850.032.18975429
GTCCGCA276300.032.1578934
ATCTCGT270850.032.0491442
TCACGTC278500.032.0385430
TCCGCAC282450.031.48923535
TCGGAAG287850.031.4136543
TCTCGTA277900.031.3404743
ATCGGAA288850.031.2711412
ACGTCTG287950.031.07815615
CACGTCT288500.031.07228514
CCGCACA287450.030.99507336
CACACGT293600.030.6820912
AGATCGG71150.030.5145111
CGTCCGC293300.030.36898833
Produced by FastQC (version 0.11.5)