FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44034974
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA476930.10830709244883396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT62550.031.172644
TTGAACG88300.026.0355837
TCGAACG90300.024.45884940
TATCTCG85700.022.38338541
GAACGCT101400.022.3460189
ACGGTAA101100.021.81138244
TCGACTA39150.021.69812444
AACGCTG105450.021.44605410
GATCGGA94200.021.2398761
GTCGAAC104450.021.20855539
CGAACGG106050.020.8470941
AGTCGAA107300.020.7272438
GAACGGT108700.020.44004642
AACGGTA108000.020.38925643
TGAACGC114650.020.0134538
GCCTAAC112550.019.58442324
ATTGAAC130800.019.0392766
ACACGTC109000.018.40589313
AAGTCGA122800.018.3617537
GGCCTAA125100.017.90107223
Produced by FastQC (version 0.11.5)