FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17195809
Sequences flagged as poor quality0
Sequence length57
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTC547210.3182228879141423TruSeq Adapter, Index 6 (100% over 57bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG74400.036.741543
CGTATGC76900.035.54704344
CTCGTAT77100.035.3556142
TATGCCG78150.035.01140646
TATCTCG82050.033.3159739
GATCGGA84900.032.7754061
CGCCAAT85100.032.48158633
AGTCACG87850.031.46498928
ACACGTC90600.030.84731513
GTCACGC90750.030.29081329
ATCTCGT93050.029.51451140
ATGCCGT93550.029.4659747
ACGTCTG95650.029.05889515
TCTCGTA94400.028.95736741
ACGCCAA96200.028.8397432
GTATGCC95600.028.78076745
ATCGGAA97650.028.5686822
TCGGAAG99500.028.0375063
CACGTCT100050.027.98475614
GCCGTCT99350.027.66908349
Produced by FastQC (version 0.11.5)