FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15547264
Sequences flagged as poor quality0
Sequence length57
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTC3095491.9910191272239284TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCT2184181.404864547228374TruSeq Adapter, Index 19 (97% over 40bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAG217330.13978665313716934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG356350.045.8055231
GCCGTCT269650.042.460551
TTGAACG52550.031.1528137
AACGCTG54450.029.78476710
TCGTATG559750.028.9324946
CTCGTAT558900.028.91261745
GAACGCT57100.028.6257519
ATCTCGT569450.028.43508143
ACGATCT570850.028.24482740
GATCGGA580400.028.1319242
GATCTCG574050.028.11394342
TGGTCGC21700.028.0853674
TATGCCG577000.028.0852949
CGTATGC577700.028.0511747
AACGATC576250.028.01112639
TGAAACG578700.028.00269736
TCTCGTA580950.027.8458744
GAAACGA582350.027.80090937
CGATCTC580100.027.7856641
TCGGAAG590350.027.649054
Produced by FastQC (version 0.11.5)