FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58674202
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1488160.2536310591833869TruSeq Adapter, Index 25 (97% over 44bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA909350.15498293440786803No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA692560.11803483922968394TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT195200.035.96082344
TTGAACG166750.026.1050687
TCGACTA65700.026.04934944
AACGCTG174450.024.60040710
GATCGGA298300.024.3971481
TCGAACG174300.024.21893740
TATCTCG289550.024.06794541
AGATCGG140300.023.6985971
ATCTCGT298550.023.2174242
GAACGCT186300.023.2002939
ACACGTC310500.022.9810413
ACGGTAA183850.022.88937644
GTCGAAC188850.022.39775839
GAACGGT189250.022.3641242
ATTGAAC204900.022.3073756
AGTCGAA193350.021.91071938
ACGTCTG327500.021.82848515
TCGGAAG331350.021.710593
CACGTCT334550.021.42766614
ACTGATA334050.021.3958634
Produced by FastQC (version 0.11.5)