FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15510694
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT543480.3503905112176154TruSeq Adapter, Index 13 (97% over 40bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA249190.1606568990401074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA87350.030.355251
CTCGTAT85750.030.09357544
TTGAACG43750.029.0125057
TCGGAAG95350.027.6650073
TCGAACG44800.027.6461540
ATCGGAA100300.026.4354692
AACGCTG48300.025.82391210
GAACGCT48500.025.8081329
ACACGTC104200.025.31280713
TCGACTA16650.025.23649244
ACGGTAA49000.025.18701244
GAACGGT49750.024.89454542
GTCGAAC50250.024.51637539
ATCTCGT106800.023.87267542
ACGTCTG111200.023.79850815
AGTCGAA52300.023.68159938
CGAACGG52650.023.60773741
CACGTCT112800.023.49994714
ATTGAAC55900.023.4936246
GCACACG113350.023.34695211
Produced by FastQC (version 0.11.5)