FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35208194
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2042460.5801092779709178TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG924900.26269453071066357TruSeq Adapter, Index 5 (100% over 49bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA650260.18468996166062934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC272750.036.0498944
AGATCGG144700.031.3382721
TTGAACG104100.028.571937
GATCGGA365500.027.327291
TCGTATG369200.026.62209943
CTCGTAT370900.026.3221142
ACACGTC377450.026.3023713
AACGCTG112950.025.90410410
TCGGAAG387100.025.6982383
ACGTCTG390500.025.34451115
CACGTCT395000.025.13374514
TCGAACG115150.024.9690440
GAACGCT118350.024.9266369
ATCTCGT384200.024.90131240
ATCGGAA399100.024.8819052
CGTCTGA404750.024.45224616
CACACGT407850.024.39106812
GCACACG409700.024.33999311
GATCTCG403050.023.7312639
ATTGAAC131300.023.5242636
Produced by FastQC (version 0.11.5)