FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37528339
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1442590.3844001728933434TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA622120.16577339061022658TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT194800.034.9051644
CATACGA53150.033.98298615
GCATACG55200.032.96006814
ATACGAG53650.032.4322416
CGGCATA58000.031.78613312
GATCGGA272050.025.8203351
TCGAACG63700.025.7617540
AGATCGG121450.025.0988141
TTGAACG70250.024.487117
ACACGTC289750.024.25119613
ATCGGAA296350.023.7425772
ACGTCTG296000.023.6945415
TCGGAAG297350.023.638443
ACGGTAA71450.023.1290544
AATCTCG288600.023.09519841
ATCTCGT291000.022.97275542
CACGTCT305950.022.967114
TCTCGTA294250.022.80125643
CGTCTGA309000.022.70922916
ACGAGAT74850.022.5116418
Produced by FastQC (version 0.11.5)