FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41338606
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT583840.14123359650782613TruSeq Adapter, Index 22 (97% over 40bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA466630.11287995536182328No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA447640.10828618652501248TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT82650.034.29151544
TTGAACG83400.026.270757
TCGAACG87150.024.5595240
AGATCGG90350.023.8574371
AACGCTG94400.023.00212510
CGTACGT123750.022.80720934
GAACGCT96850.022.6024049
TACGTAA126200.022.503736
ACGGTAA96200.022.23334744
GTCGAAC99500.021.55539539
ACCGTAC131200.021.54710432
ACGTAAT130150.021.43197337
CGTAATC129350.021.36044338
GTACGTA133600.021.29016935
AACGGTA100900.021.16905443
GAACGGT103750.020.69355642
AGTCGAA105100.020.59583738
CGAACGG103250.020.58071541
GCCTAAC105350.020.50594324
TGAACGC108500.020.375848
Produced by FastQC (version 0.11.5)