FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13133964
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT4526153.446141621828718TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA1935541.4736906542457402TruSeq Adapter, Index 15 (97% over 40bp)
CAAGCAGAAGACGGCATACGAGATTCTGACATGTGACTGGAGTTCAGACG486920.37073346630156745RNA PCR Primer, Index 40 (96% over 29bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA368070.28024288782883827No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA143160.10899984193652426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA107900.040.97943512
ATACGAG102150.040.9604916
CATACGA106000.040.92550715
GCATACG107250.040.5715914
AGACGGC117200.038.0655179
ACGAGAT105200.037.99550618
CTCGTAT555900.037.91287244
ACGGCAT116950.037.86473511
AAGACGG119500.037.3512848
GGCATAC118800.037.25671813
GACGGCA120100.037.12804810
GAAGACG123400.036.45667
AGATCGG256800.035.699391
CGAGATT110700.035.49305719
ATGTGAC110550.033.8687231
GAGATTC118350.033.1627920
TACGAGA126250.032.5664917
TTGAACG56750.031.5929187
TCAGACG92250.031.55086744
GACATGT121500.031.43243628
Produced by FastQC (version 0.11.5)